Nucleic Acid Extraction Ko Tsabtace Kit
Nucleic Acid Ecire Or Tsarkakewa Kitko adana a -20 ℃. Ya kamata a yi jigilar samfurin ta amfani da 0 ℃ curling.
Introduction
Na'urar Ciro Acid Nucleic Acid Ko Tsabtace (Hanyar Magnetic Beads) an tsara shi don tsarkakewa ta atomatik na RNA da DNA daga ruwan jiki (kamar swabs, plasma, serum) ta amfani da kayan aikin cire acid nucleic mai sarrafa kansa. Fasaha barbashi-Magnetic yana ba da DNA/RNA mai inganci wanda ya dace da amfani kai tsaye a aikace-aikacen ƙasa kamar haɓakawa ko wasu halayen enzymatic.
Application Range
Dukkanin jinin, plasma, serum da sauran samfuran nama an lalata su kai tsaye kuma an narkar da su. An zaɓi acid ɗin nucleic da aka saki ta hanyar beads na magnetic super paramagnetic nanometer. Sa'an nan kuma an cire furotin, ions gishiri na inorganic da ƙazantattun kwayoyin halitta ta hanyar wankewa.
Kit Contents
| Cat. A'a. | Saukewa: YXN-VIRAL01-32A-BR | Abubuwan da aka gyara | ||
| -50A | - 100 A | |||
| Girman | 32 Tes | 50 Gwaji | Gwaji 100 | |
| Lysis Buffer | 96 rijiyar riga-kafi ed Faranti guda 2 | ml 25 | ml 50 | Surfactant da Tris |
| Wanke Buffer I | ★15ml | ★30ml | Maganin gishiri mai girma | |
| Wanke Buffer Il | ★6ml*2 | ★12ml*2 | Maganin ƙarancin gishiri | |
| Elution Buffer | ml 10 | ml 20 | Maganin ƙarancin gishiri | |
| MagaBio Reagent | 1.0 ml | 2.0ml ku | Magnetic barbashi | |
| Littafin Jagora (=YXN-VIRAL01-32A-BR) | 1 | 1 | 1 | |
| Notes:DominYXN-VIRAL01-32A-BR-50A,ƙara 15mL Absolute ethanol zuwa ★15mL Wash Buffer I kafin amfani; ƙara 24mL Absolute ethanol zuwa ★6mL Wash buffer Il kafin amfani. | ||||
| DominYXN-VIRAL01-32A-BR-100A, ƙara 30mL Absolute ethanol zuwa ★30mL Wash Buffer I kafin amfani; ƙara 48mL Absolute ethanol zuwa ★12mL Wash buffer Il kafin amfani.【Reagents da za a shirya ta mai amfani】 Da fatan za a shirya cikakken ethanol (analytical grade) da kanka. | ||||
Storage Condittions
Bayan isowar kit ɗin, za'a iya adana kayan aikin a cikin zafin jiki (15 - 25 ° C). Reagents sun tsaya tsayin daka har zuwa shekara guda daga ranar masana'anta.
Sample Requirements
1. Samfurin aiki: Swabs, plasma, serum da jini duka da dai sauransu.
2. Samfurin ajiya da sufuri: Ya kamata a gwada samfurin nan da nan
Materials kuma Devices Required but Not Provided
1. Safofin hannu marasa foda da za a iya zubar da su
2. Kwangilar Biohazard
3. Fensir ko per
Procedure
Abubuwan da ke biyowa suna amfani da samfurin tsiri swab ruwan shafa fuska a matsayin misali don yin bayani a taƙaice matakan aiki na reagent na hakar akan kayan aikin haɓakar kwayoyin nucleic acid.Bioer NPA-32PkoSMART 32. Don wasu nau'ikan samfurin, da fatan za a koma zuwa littafin mai amfani. Hakanan abokan ciniki za su iya sarrafa shi bisa ga siyan gwaji:
1. Reagent Shiri
a. DominYXN-VSaukewa: B03-32A-50A kuma Saukewa: YXN-VB03-32A-100A
Ƙara 500uL Lysis Buffer zuwa shafi na 1 da 7 na 2.2mL 96-zurfin rijiyar farantin, 500uL Wash Buffer I zuwa shafi na 2 da 8, 500uL Wash Buffer II zuwa shafi na 3, 4 da 9,10; 70uL Elution Buffer zuwa shafi na 5 da 11, 180uL tsarkakakken ruwa da 20uL MagaBio Reagent zuwa shafi na 6 da 12 (ya kamata a gauraya ƙwanƙolin maganadisu sosai kafin amfani).
b. DominSaukewa: YXN-VB03-32A
Sanya reagents 96 da aka riga aka shirya sosai a zafin daki. Girgiza farantin rijiyar 96 a juye har sau uku, sannan a yayyage jakar filastik. Sanya reagent ɗin da aka riga aka shirya don ƴan daƙiƙa (ko lilo da hannu sau ƴan) don gujewa reagent manne da bangon bututu. Yage fim ɗin foil na aluminum na farantin rijiyar 96 kuma gano alkiblar farantin (maganin maganadisu a shafi na #6 da #12),
2.Misali Hakowa
1. Ƙara samfurin 300uL zuwa ginshiƙan farantin rijiyar 96 # 1 da # 7, don Allah ku guje wa gurɓataccen giciye,
2. Sanya farantin rijiyar 96 mai zurfi zuwa kayan aiki, shigar da tukwici 8-strip akan kayan aiki,
3. Gudanar da shirin bisa ga matakai masu zuwa.
4. Bayan an gama tsarkakewa ta atomatik, canja wurin buffer buffer a cikin ginshiƙan 5 da 11 zuwa bututun centrifuge mai tsabta na antinuclear 0.5mL; Idan ba a yi amfani da shi nan da nan ba, da fatan za a adana a -20 ° C.
Performance Characteristics
1. Samfurin da aka fitar ana gano shi ta hanyar haɓakar HBV DNA gano reagent don isa hankali na 10 IU/ml. Ana gano samfurin da aka fitar ta hanyar babban ji na HCV RNA reagent ganowa don isa hankali na 50 IU/ml.
2. Zaɓi samfuran 4 (samfurin ƙwayar ƙwayar cuta / plasma, samfurin swab nasopharyngeal, samfurin exfoliated cell na mahaifa), kowane samfurin yana diluted sau 10 tare da 3 gradients (ciki har da ainihin samfurin jimlar 4 maida hankali), ta yin amfani da ƙwararrun reagents da wakilai na gwaji don gano ciki. Maganar kwayar halitta bisa ga umarnin samfur, kuma ƙimar Ct na kowane tsari ya bambanta da ƙasa da 1.
| Step | To Wuri | Program Suna | Waitting Time(min:SS) | Mixing Time(mcikin:SS) | Magnet Time(min:SS) | Arashin jin daɗi | Sfeda | Vmai girma tatus (μL) | Tdaular |
| 1 | 1 | Lysis | 0:00 | 2:00 | 0:00 | F | 700 | 80 | |
| 2 | 6 | Bbakin ciki | 0:00 | 0:15 | 0:15 | √ | F | 200 | |
| 3 | 1 | Bind | 0:00 | 3:00 | 0:45 | √ | F | 700 | |
| 4 | 2 | Wash1 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 5 | 3 | Wash2 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 6 | 4 | Wash3 | 0:00 | 0:30 | 0:30 | √ | F | 500 | |
| 7 | 5 | Haske | 2:00 | 2:30 | 0:30 | F | 70 | 80 | |
| 8 | 6 | Yi watsi da | 0:00 | 0:15 | 0:00 | F | 200 |
Safety
1. JANARAL TSIRA.
Yin amfani da wannan samfurin ta hanyar da ba a kayyade ba a cikin takaddun mai amfani na iya haifar da rauni ko lalacewa ga kayan aiki ko na'ura. Tabbatar cewa duk wanda ke amfani da wannan samfur ya karɓi umarni a cikin ayyukan aminci gabaɗaya don dakunan gwaje-gwaje da bayanan aminci da aka bayar a cikin wannan takaddar.
1.1 Kafin amfani da kayan aiki ko na'ura, karanta kuma ku fahimci amincin bayanan da aka bayar a cikin takaddun mai amfani da mai ƙira ko na'urar ya bayar.
1.2 Kafin sarrafa sinadarai, karanta kuma ku fahimci duk takaddun bayanan aminci (SDSs) da suka dace kuma yi amfani da kayan kariya masu dacewa (safofin hannu, riguna, kariyan ido, da sauransu). Don samun SDSs, duba sashin "Takardu da Tallafawa" a cikin wannan takarda.
2. Chemical aminci
JAMA'A CUTAR SAUKI. Don rage hatsarori, tabbatar da cewa ma'aikatan dakin gwaje-gwaje sun karanta kuma suyi aiki da ƙa'idodin aminci na gabaɗaya don amfani da sinadarai, ajiya, da sharar da aka bayar a ƙasa, kuma tuntuɓi SDS masu dacewa don takamaiman takamaimai da umarni: Karanta kuma ku fahimci Tabbatattun Bayanan Tsaro (SDS) da sinadarai ta samar. masana'anta kafin ka adana, rike, ko aiki tare da kowane sinadarai ko abubuwa masu haɗari.
2.1 Rage hulɗa da sunadarai. Sanya kayan kariya masu dacewa lokacin sarrafa sinadarai (misali, gilashin tsaro, safar hannu, ko tufafin kariya).
2.2 Rage shakar sinadarai. Kar a bar kwantena sinadarai a bude. Yi amfani kawai tare da isassun iska (misali, murfi).
2.3 Bincika akai-akai don yatsanun sinadarai ko zubewa. Idan zubewa ko zubewa ta faru, bi hanyoyin tsabtace masana'anta kamar yadda aka ba da shawarar a cikin SDS.
2.4 Hannun sharar sinadarai a cikin hurumin hayaƙi.
2.5 Tabbatar da amfani da kwantena na sharar gida da na sakandare. (Sharar farko
kwandon yana riƙe da sharar nan take. Kwantena na biyu ya ƙunshi zubewa ko zubewa daga babban akwati. Duk kwantena biyu dole ne su dace da kayan sharar gida kuma su cika buƙatun tarayya, jihohi, da na gida don ajiyar kwantena.).
2.6 Bayan zubar da kwandon shara, rufe shi da hular da aka tanadar.
2.7 Halaye (ta hanyar bincike idan ya cancanta) sharar da takamaiman aikace-aikace, reagents, da abubuwan da ake amfani da su a cikin dakin gwaje-gwajen ku.
2.8 Tabbatar cewa an adana, canjawa wuri, jigilar kaya, da zubar da shi bisa ga duk ƙa'idodin gida, jiha/lardi, da/ko na ƙasa.
2.9 Abubuwan rediyoaktif ko abubuwan haɗari masu haɗari na iya buƙatar kulawa ta musamman, kuma ana iya amfani da iyakokin zubarwa.
3.Biological hadari aminci
Mai yuwuwar Biohazard. Dangane da samfuran da aka yi amfani da su akan wannan kayan aikin, ana iya ɗaukar saman a matsayin hazari. Yi amfani da hanyoyin ƙazanta da suka dace lokacin aiki tare da haɗari masu haɗari.
BIYOHAZARD. Samfuran halittu irin su kyallen takarda, ruwan jiki, masu kamuwa da cuta, da jinin mutane da sauran dabbobi suna da damar yada cututtuka masu yaduwa. Bi duk dokokin gida, jiha/lardi, da/ko na ƙasa. Saka kayan kariya masu dacewa, wanda ya haɗa amma ba'a iyakance ga: kayan kariya na ido, garkuwar fuska, sutura/kawul na lab, da safar hannu. Dole ne a gudanar da duk ayyukan a cikin kayan aikin da ya dace ta amfani da kayan aikin aminci da suka dace (misali, na'urori masu ɗaukar jiki). Ya kamata a horar da ɗaiɗaikun bisa ga ƙa'idodi masu dacewa da buƙatun kamfani/ma'aikata kafin yin aiki da abubuwan da ke iya kamuwa da cuta.
Karanta ku bi jagororin da suka dace da/ko buƙatun tsari a cikin masu zuwa:
A cikin Amurka: Jagororin Sashen Lafiya na Amurka da Sabis na Jama'a da aka buga a cikin Biosafety a cikin Dakunan gwaje-gwajen Microbiological da Biomedical da aka samu a: www.cdc.gov/biosafety.
Ma'aunin Tsaro da Kiwon Lafiyar Ma'aikata, Magungunan Jini (29 CFR§1910.1030), da aka samu a:
www.access.gpo.gov/nara/cfr/waisidx_01/29cfr1910a_01.html
Ka'idojin Shirin Biosafety na kamfanin ku/ma'aikatar ku don aiki tare da / sarrafa kayan yuwuwar kamuwa da cuta. Ana samun ƙarin bayani game da jagororin biohazard a: www.cdc.gov.
A cikin EU: Bincika ƙa'idodin gida da ƙa'idodi game da rigakafin ƙwayoyin cuta da kiyaye lafiyar halittu kuma koma zuwa mafi kyawun ayyuka da aka buga a cikin Littafin Lantarki Biosafety na Hukumar Lafiya ta Duniya (WHO), bugu na uku, wanda aka samu a: www.who.int/csr/resources/publications /biosafety/WHO_CDS_CSR_LYO_200 4_11/ha/.
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