Sabbin Kayan Gane Acid Nucleic Acid (SARS-Cov-2).

New Coronavirus(SARS-Cov-2) Nucleic Acid Detection Kit

(Fluorescent RT-PCR Probe Method) Product Manual

【Produkt name 】Sabuwar Coronavirus (SARS-Cov-2) Kit ɗin Gano Acid Nucleic (Tsarin Binciken Fluorescent RT-PCR)

【Packaging specfications 】Gwaje-gwaje 25/Kit

【Intended usshekaru】

Ana amfani da wannan kit ɗin don gano ƙimar nucleic acid daga sabon coronavirus a cikin swabs na nasopharyngeal, swabs oropharyngeal (maƙogwaro), swabs na baya, tsakiyar turbinate swabs, wankin hanci da masu neman hanci daga mutanen da ake zargi da COVID19 ta hanyar mai ba da kiwon lafiya. Gano kwayoyin ORF1ab da N na sabon coronavirus za a iya amfani da su don ƙarin bincike da kuma lura da cututtukan cututtukan sabon kamuwa da cuta na coronavirus.

【Principles of the procedure 】

An tsara wannan kit ɗin don takamaiman bincike na TaqMan da takamaiman abubuwan da aka tsara don sabon coronavirus (SARS-Cov-2) ORF1ab da jerin jerin kwayoyin N. Maganin amsawar PCR yana ƙunshe da saiti 3 na ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun bayanai da bincike mai kyalli don takamaiman gano maƙasudi, kuma ana amfani da ƙarin saitin ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun abubuwa da bincike mai kyalli a matsayin daidaitaccen daidaitaccen abin cikin kit ɗin don gano ƙwayoyin halittar gida na endogenous.

Ka'idar gwajin ita ce takamaiman bincike na fluorescent yana narkewa kuma an lalata shi ta hanyar aikin exonuclease na Taq enzyme a cikin amsawar PCR, ta yadda ƙungiyar masu ba da labari da ƙungiyar masu walƙiya ta rabu, ta yadda tsarin saka idanu mai kyalli zai iya samun kyalli. sigina, sa'an nan Ta hanyar haɓaka haɓakar haɓakawa na PCR, siginar kyalli na binciken ya kai ga ƙimar ƙimar da aka saita - ƙimar Ct (Kofin Zagaye). Idan babu amplicon da aka yi niyya, ƙungiyar masu ba da rahoto na binciken suna kusa da ƙungiyar masu kashewa. A wannan lokacin, canja wurin makamashi mai haske yana faruwa, kuma hasken ƙungiyar masu ba da rahoto ya ƙare ta ƙungiyar quenching, ta yadda na'urar PCR ba za ta iya gano siginar mai kyalli ba.

Don saka idanu da yin amfani da reagents a lokacin gwajin, kit ɗin yana sanye take da ingantattun sarrafawa da mara kyau: ingantaccen iko yana ƙunshe da maƙasudin rukunin yanar gizon recombinant plasmid, kuma mummunan iko shine Distilled ruwa, wanda ake amfani da shi don saka idanu gurɓataccen muhalli. Ana ba da shawarar saita ingantaccen iko da kuma mummunan iko lokaci guda lokacin gwaji.

【Main components 】

Lura:(1) Abubuwan da ke cikin na'urorin batch daban-daban ba za a iya haɗa su ko musanya su ba.

(2) Shirya naka reagent: Nucleic acid hakar kit.

【Storage condittions kuma expiration date 】

For BST-SARS-25:Transport da adana a -20 ± 5 ℃ na dogon lokaci.

For BST-SARS-DR-25:Transport a dakin da zafin jiki. Adana a -20 ± 5 ℃ na dogon lokaci.

Guji maimaita daskarewa-narkewa. An saita lokacin aiki na ɗan lokaci na watanni 12.

Dubi lakabin ranar ƙira da amfani.

Bayan budewa na farko, ana iya adana reagent a -20 ± 5 ° C don ba fiye da wata 1 ba ko har zuwa ƙarshen lokacin reagent, duk ranar da ta zo ta farko, don guje wa maimaita daskare-narke hawan keke, da adadin daskarewar reagent. -zargin narke kada ya wuce sau 6.

【Applicable instrument】ABI 7500, SLAN-96P, Roche-LightCycler-480.

【Sample requirements 】

1.Amfani nau'in samfurin: Cire maganin nucleic acid.

2.Sample ajiya da sufuri: Adana a-20 ± 5 ℃ don watanni 6. Daskare da narke samfurori ba fiye da sau 6 ba.

【Testing method】

1.Nucleic acid extraction

Zaɓi kayan cirewar acid nucleic mai dacewa don cire ƙwayar nucleic acid, kuma bi umarnin kit ɗin daidai. Ana ba da shawarar yin amfani da kayan cirewar acid Nucleic da kayan tsarkakewa wanda Yixin Bio-Tech (Guangzhou) Co., Ltd. ke samarwa ko makamancin kayan tsarkakewa na acid nucleic.

2.  Reaction reagent preparation

2.1 For BST-SARS-25:

( 1) Cire SARS-Cov-2 Mix da Enzyme Mix , gaba ɗaya narke a cikin yanayin zafin jiki sosai ta hanyar na'urar Vortex sannan a taƙaice a taƙaice.

(2) 16.5uL Enzyme Mix an ƙara shi zuwa 358.5uL SARS-Cov-2 Mix kuma an gauraye shi sosai don samun maganin abin da ya gauraya.

(3) Shirya 0.2 ml PCR octal bututu mai tsabta kuma yi masa alama tare da 15uL na maganin gauraye na sama a kowace rijiya.

(4) Ƙara 15 μL na tsaftataccen maganin nucleic acid, ingantaccen iko da iko mara kyau, kuma a hankali rufe murfin bututun octal.

(5) Mix da kyau ta hanyar juye juye, da sauri a ɗaga santsi don tattara ruwan da ke ƙasan bututu.

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