COVID-19 IgM/IgG Antibody Detection Kit

COVID-19 IgM/IgG Antibody Detection Kit

(Colloidal Gold Immunochromatography Method) Product Manual

【PRUDUCT NAME】 COVID- 19 IgM/IgG Kayan Ganewar Jikin Jiki (Tsarin Immunochromatography Colloidal Gold) 【PACKAGING SPECIFICATIONS】 Gwaji 1/Kit , Gwaji 10/Kit

【ABSTRACT】

Novel coronaviruses na cikin nau'in β. COVID-19 cuta ce mai saurin kamuwa da numfashi. Mutane gabaɗaya suna da sauƙi. A halin yanzu, marasa lafiya da suka kamu da cutar sankara ta coronavirus sune babban tushen kamuwa da cuta; Mutanen da suka kamu da asymptomatic suma suna iya zama tushen kamuwa da cuta. Dangane da binciken cututtukan cututtuka na yanzu, lokacin shiryawa shine kwanaki 1 zuwa 14, galibi daga kwanaki 3 zuwa 7. Babban bayyanar cututtuka sun haɗa da zazzabi, gajiya da bushewar tari. Ana samun cunkoso na hanci, hanci, ciwon makogwaro, myalgia da gudawa a wasu lokuta.

【EXPECTED USAGE】

Wannan kit ɗin ya dace don gano ƙimar COVID-19 ta hanyar gano ƙwayoyin rigakafin 2019-nCoV IgM/IgG a cikin jini, plasma, ko duka jini. Alamomin kamuwa da cuta na yau da kullun tare da 2019-nCoV sun haɗa da alamun numfashi, zazzabi, tari, gajeriyar numfashi, da dyspnea. A cikin lokuta masu tsanani, kamuwa da cuta na iya haifar da ciwon huhu, matsanancin ciwo na numfashi, gazawar koda, har ma da mutuwa. Ana iya fitar da 2019 nCoV ta hanyar ɓoyewar numfashi ko kuma yada ta ta hanyar ruwa na baki, atishawa, saduwa ta jiki, da ta digon iska.

【PRINCIPLES OF THE PROCEDURE】

Ka'idar immunochromatography na wannan kit: rabuwa da aka gyara a cikin cakuda ta hanyar matsakaici ta hanyar amfani da ƙarfin capillary da ƙayyadaddun da sauri dauri na antigen zuwa antigen. Wannan gwajin ya ƙunshi kaset guda biyu, kaset na IgG da kaset na IgM.

Don YXI-CoV- IgM&IgG-1 da YXI-CoV- IgM&IgG- 10: A cikin kaset na IgM, busassun matsakaici ne wanda aka lulluɓe shi daban tare da layin gwaji na 2019-nCoV (layin gwajin “T”) da goat anti-mouse. polyclonal antibodies (layin kula da "C"). The colloidal zinariya-labeled antibodies, linzamin kwamfuta anti-human IgM (mIgM) ne a cikin saki pad sashe. Da zarar diluted serum, plasma, ko dukan jini aka shafa a sample pad sashe (S), mIgM antibody zai ɗaure zuwa 2019- nCoV IgM rigakafi idan suna nan, suna samar da hadaddun mIgM-IgM. Hadaddiyar mIgM-IgM daga nan za ta wuce ta tace nitrocellulose (NC filter) ta hanyar aikin capillary. Idan 2019-nCoV IgM antibody yana nan a cikin samfurin, layin gwajin (T) za a ɗaure shi da hadaddun mIgM-IgM kuma ya haɓaka launi. Idan babu maganin rigakafi na 2019-nCoV IgM a cikin samfurin, mIgM kyauta ba zai ɗaure layin gwajin (T) kuma babu launi da zai haɓaka. MIgM na kyauta zai ɗaure zuwa layin sarrafawa (C); wannan layin sarrafawa ya kamata a bayyane bayan matakin ganowa kamar yadda wannan ya tabbatar da cewa kit ɗin yana aiki da kyau.A cikin kaset na IgG, busassun matsakaici ne wanda aka lulluɓe shi daban tare da linzamin kwamfuta anti-human IgG (layin gwajin "T") da Rabbit. antichicken IgY antibody (layin sarrafawa "C"). Kwayoyin rigakafi masu alamar zinari, 2019-nCoV antigen da kaji IgY antibody suna cikin sashin kushin saki. Da zarar an diluted serum, plasma, ko duka jini ana shafa shi a sashin kushin samfurin (S), da

colloidalgold-2019-nCoV recombinant antigen zai ɗaure ga 2019-nCoV IgG antibodies idan suna nan, samar da colloidalgold-2019-nCoV recombinant antigen-IgG hadaddun. Sa'an nan hadaddun zai matsa a kan nitrocellulose filter (NC filter) ta hanyar aikin capillary. Idan 2019-nCoV IgG antibody yana cikin samfurin, layin gwajin (T) za a ɗaure shi ta colloidalgold-2019-nCoV recombinant antigen-IgG hadaddun da haɓaka launi. Idan babu 2019-nCoV IgG antibody a cikin samfurin, free colloidalgold-2019-nCoV antigen recombinant ba zai ɗaure da gwajin line (T) kuma babu wani launi da zai tasowa. The free colloidal zinariya-kaza antibody IgY zai ɗaure zuwa kula line (C); wannan layin sarrafawa ya kamata a bayyane bayan matakin ganowa saboda wannan yana tabbatar da cewa kit ɗin yana aiki yadda yakamata.

Don YXI-CoV-IgM&IgG-02-1 da YXI-CoV- IgM&IgG-02-10: Ka'idar immunochromatography na wannan kit: Rarraba abubuwan da aka haɗa a cikin cakuda ta hanyar matsakaici ta hanyar amfani da ƙarfin capillary da ƙayyadaddun da sauri dauri na antigen zuwa antigen. Kit ɗin Ganewar rigakafin COVID-19 IgM/IgG shine ingantaccen rigakafin rigakafi na tushen membrane don gano ƙwayoyin IgG da IgM ga SARS-CoV-2 a cikin jini gaba ɗaya, jini ko samfuran plasma. Wannan gwajin ya ƙunshi abubuwa biyu, bangaren IgG da bangaren IgM. A cikin ɓangaren IgG, an rufe IgG anti-dan Adam a cikin yankin layin gwajin IgG. Yayin gwaji, samfurin yana amsawa da ƙwayoyin antigen-mai rufi na SARS-CoV-2 a cikin kaset ɗin gwajin. Cakuda sannan ya yi ƙaura ta gefe tare da membrane chromatographically ta hanyar aikin capillary kuma yana amsawa tare da IgG na ɗan adam a cikin layin gwajin IgG, idan samfurin ya ƙunshi ƙwayoyin rigakafi na IgG zuwa SARSCoV-2. Layi mai launi zai bayyana a yankin layin gwajin IgG sakamakon wannan. Hakazalika, an rufe IgM na ɗan adam a cikin yankin layin gwajin IgM kuma idan samfurin ya ƙunshi ƙwayoyin rigakafi na IgM zuwa SARS-CoV-2, hadaddun samfurin haɗin gwiwar yana amsawa da IgM na ɗan adam. Layi mai launi ya bayyana a yankin layin gwajin IgM a sakamakon haka. Don haka, idan samfurin ya ƙunshi ƙwayoyin rigakafin SARS-CoV-2 IgG, layin launi zai bayyana a yankin layin gwajin IgG. Idan samfurin ya ƙunshi ƙwayoyin rigakafin SARS-CoV-2 IgM, layin launi zai bayyana a yankin layin gwajin IgM. Idan samfurin bai ƙunshi ƙwayoyin rigakafin SARS-CoV-2 ba, babu wani layi mai launi da zai bayyana a cikin ɗayan layin gwajin, yana nuna mummunan sakamako. Don yin aiki azaman tsarin sarrafawa, layi mai launi koyaushe zai bayyana a cikin yankin layin sarrafawa, yana nuna cewa an ƙara ƙarar samfurin da ya dace kuma wicking membrane ya faru.

【MAIN COMPONENTS】

Lura: Abubuwan da ke cikin kayan batch daban-daban ba za a iya haɗa su ko musanya su ba.

【MATERIALS TO BE PROVIDE BY USER】

•Tashin barasa

•Alurar shan jini

【STARBIYYA KUMA EXPIRATION】

Ajiye kayan a cikin sanyi da bushe wuri a 2 - 25 ° C.

Kar a daskare.

Kayan da aka adana da kyau suna aiki na tsawon watanni 12.

【SAMPLE REQUIREMENTS】

Assay ya dace da jinin mutum, plasma, ko duka samfuran jini. Ya kamata a yi amfani da samfurori da wuri-wuri bayan tattarawa. Tarin jini da jini: Ya kamata a rabu da jini da plasma da wuri-wuri bayan an tattara jini don guje wa hemolysis.

【SAMPLE PRESERVATION】

Ya kamata a yi amfani da jini da plasma da wuri-wuri bayan tattarawa kuma a adana su a 2-8 ° C na kwanaki 7 idan ba a yi amfani da su nan da nan ba. Idan ana buƙatar ajiya na dogon lokaci, da fatan za a adana a -20 ° C na lokaci ƙasa da watanni 2. A guji maimaita daskarewa da narkewa.

Ya kamata a gwada samfurin jini gabaɗaya ko na gefe a cikin sa'o'i 8 bayan tattarawa.

Ba za a yi amfani da haemolysis mai tsanani da samfuran jinin lipid ba don ganowa.

【TESTING METHOD】

Don YXI-CoV- IgM&IgG- 1 da YXI-CoV- IgM&IgG- 10:

Karanta umarnin a hankali kafin amfani. Kawo tsiri na Gwaji, Samfurin diluent bututu, da samfurin zuwa zafin daki kafin gwaji.

1. Ƙara 50 µl na Jini gabaɗaya ko na gefe ko 20 µl Serum da plasma zuwa Samfurin diluent tube sannan a gauraya sosai. Ƙara 3-4 saukad da zuwa sashin kushin samfurin.

2. Bar a dakin da zafin jiki na minti 5 don lura da sakamakon. Sakamako da aka auna bayan mintuna 5 basu da inganci kuma yakamata a jefar dasu. Don YXI-CoV- IgM&IgG-02- 1 da YXI-CoV- IgM&IgG-02- 10:

Karanta umarnin a hankali kafin amfani. Kawo tsiri na Gwaji, Samfurin diluent bututu, da samfurin zuwa zafin daki kafin gwaji.

1. Ƙara 25µl na Duka ko jini na gefe ko 10µl Serum da plasma zuwa Samfurin diluent tube kuma gauraya sosai. Ƙara 4 saukad da zuwa samfurin kushin

sashe.

2. Bar a dakin da zafin jiki na minti 5 don lura da sakamakon. Sakamako da aka auna bayan mintuna 5 basu da inganci kuma yakamata a jefar dasu.

【[INTERPRETATION OF GWADA RESULTS】